mtrA [2019-02-24 17:45:28]

methylthioribose transporter

locus: BSU07340

pI: 9.82

mw: 48.74 kDa

protein length: 461 aa

gene length: 1386 bp

function: uptake of methylthioribose

product: methylthioribose transporter

essential: no

synonyms: yfnA

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.12|Other transporters]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 805,456 806,841

The protein

Catalyzed reaction/ biological activity

• uptake of methylthioribose [pubmed|29280348]

Protein family

• [SW|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|BcaP]

Structure

• [PDB|5OQT] (from Geobacillus kaustophilus, 60% identity) [pubmed|29416041]

[SW|Localization]

• cell membrane [Pubmed|18763711]

Expression and Regulation

Operons

• genes: [gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]-[gene|2E1E22349750C3EE9B6575EED30A82ED0D5F07F0|yfnB]
  description: [Pubmed|22383849]

• genes: [gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]
  description: [pubmed|22383849]

Biological materials

Mutant

• GP2379 ∆''[gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]''::''kan'', available in [SW|Jörg Stülke]'s lab
• MGNA-C231 (yfnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2229 NBRP B. subtilis, Japan]
• BKE07340 ([gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAAGTTCCTCCTAGA, downstream forward: _UP4_TAATCTCTTTTCAGCCGGCG
• BKK07340 ([gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAAGTTCCTCCTAGA, downstream forward: _UP4_TAATCTCTTTTCAGCCGGCG

Expression vector

• pGP2281: expression of ''[gene|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]'' by [SW|pBQ200] in ''B. subtilis'', available in [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP2278 (in [protein|search|pAC5]) (GP2964), available in [SW|Jörg Stülke]'s lab

References

• 18763711,29280348,29416041